diff --git a/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js b/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
index 7519e7d5898..7de76c79254 100644
--- a/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
+++ b/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
@@ -970,17 +970,6 @@ const definitions: FeatureFlagDefinitions = {
       },
       ossReleaseStage: 'none',
     },
-    animatedShouldDebounceQueueFlush: {
-      defaultValue: true,
-      metadata: {
-        dateAdded: '2024-02-05',
-        description:
-          'Enables an experimental flush-queue debouncing in Animated.js.',
-        expectedReleaseValue: true,
-        purpose: 'experimentation',
-      },
-      ossReleaseStage: 'none',
-    },
     animatedShouldSyncValueBeforeStartCallback: {
       defaultValue: true,
       metadata: {
diff --git a/packages/react-native/src/private/animated/NativeAnimatedHelper.js b/packages/react-native/src/private/animated/NativeAnimatedHelper.js
index eaaefb5b9a0..03d7f5c1c23 100644
--- a/packages/react-native/src/private/animated/NativeAnimatedHelper.js
+++ b/packages/react-native/src/private/animated/NativeAnimatedHelper.js
@@ -203,11 +203,7 @@ const API = {
   disableQueue(): void {
     invariant(NativeAnimatedModule, 'Native animated module is not available');
 
-    if (ReactNativeFeatureFlags.animatedShouldDebounceQueueFlush()) {
-      scheduleQueueFlush();
-    } else {
-      API.flushQueue();
-    }
+    scheduleQueueFlush();
   },
   disconnectAnimatedNodeFromView(nodeTag: number, viewTag: number): void {
     NativeOperations.disconnectAnimatedNodeFromView(nodeTag, viewTag);
@@ -311,10 +307,7 @@ const API = {
 
     waitingForQueuedOperations.add(id);
     queueOperations = true;
-    if (
-      ReactNativeFeatureFlags.animatedShouldDebounceQueueFlush() &&
-      flushQueueImmediate
-    ) {
+    if (flushQueueImmediate) {
       clearImmediate(flushQueueImmediate);
     }
   },
diff --git a/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js b/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
index 918b6b821aa..a0128c87b1a 100644
--- a/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
+++ b/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
@@ -4,7 +4,7 @@
  * This source code is licensed under the MIT license found in the
  * LICENSE file in the root directory of this source tree.
  *
- * @generated SignedSource<<f44477b5f5a4f7030798a61694d0332a>>
+ * @generated SignedSource<<1a333a44919efce6bb2070bae79bdf9b>>
  * @flow strict
  * @noformat
  */
@@ -31,7 +31,6 @@ export type ReactNativeFeatureFlagsJsOnly = Readonly<{
   jsOnlyTestFlag: Getter<boolean>,
   animatedDeferStartOfTimingAnimations: Getter<boolean>,
   animatedForceNativeDriver: Getter<boolean>,
-  animatedShouldDebounceQueueFlush: Getter<boolean>,
   animatedShouldSyncValueBeforeStartCallback: Getter<boolean>,
   animatedShouldUseSingleOp: Getter<boolean>,
   deferFlatListFocusChangeRenderUpdate: Getter<boolean>,
@@ -151,11 +150,6 @@ export const animatedDeferStartOfTimingAnimations: Getter<boolean> = createJavaS
  */
 export const animatedForceNativeDriver: Getter<boolean> = createJavaScriptFlagGetter('animatedForceNativeDriver', false);
 
-/**
- * Enables an experimental flush-queue debouncing in Animated.js.
- */
-export const animatedShouldDebounceQueueFlush: Getter<boolean> = createJavaScriptFlagGetter('animatedShouldDebounceQueueFlush', true);
-
 /**
  * When a useNativeDriver animation completes, syncs the JS-side AnimatedValue with the post-animation value BEFORE invoking the user-supplied start({finished}) callback. Without the flag, the callback observes the pre-animation value, which can cause downstream re-renders to read stale interpolation outputs.
  */